Gupima HFRS - Indwara ya Hemorhagashi hamwe na Syndrome yimpyiko

Amavu n'amavuko

Virusi ya Hantaan (HV) niyo ntandaro yambere itera indwara ya Hemorrhagic Fever hamwe na Syndrome yimpyiko (HFRS). HFRS n'indwara ikwirakwizwa na zoonotic ikwirakwizwa ku isi yose irangwa no kugira umuriro, kuva amaraso, no kutagira impyiko. Iyi ndwara ifite intangiriro ikabije, gutera imbere byihuse, ndetse n’impfu nyinshi, bikaba byangiza ubuzima rusange. Imbeba (nka Apodemus agrarius na Rattus norvegicus) ni ibigega nyamukuru na vectors za HV. Kwanduza abantu bibaho cyane cyane binyuze mu kirere (inkari, umwanda, amacandwe), guhura bitaziguye, cyangwa kurumwa na vector. HFRS irashobora kubaho umwaka wose, kandi abaturage muri rusange birashoboka. Nk’uko imibare ya OMS ibigaragaza, ibihugu 32 ku isi byagaragaje ko byanduye HV, aho byiganje cyane muri Aziya y'Uburasirazuba, Uburayi, na Balkans.

Antibody Markers Yandika HV

Nyuma yo kwandura HV, sisitemu yumubiri yumuntu itanga antibodi zihariye, cyane cyane HV-IgM na HV-IgG.

Ant Antibodiyite za HV-IgM: Gukora nk'ikimenyetso cya serologiya yo kwandura hakiri kare, mubisanzwe bigaragara muminsi mike ibimenyetso byatangiye, kandi ni ngombwa mugupima indwara ikabije.

Ant Antibodies za HV-IgG: Emerge nyuma kandi irashobora gukomeza kubaho, byerekana kwandura cyangwa kwandura. Kwiyongera inshuro enye cyangwa nyinshi muri antibody ya HV-IgG hagati ya serumu ikaze na convalescent ya serumu nayo isuzumwa kwandura gukabije.

Uburyo busanzwe bwo gusuzuma HV

Uburyo bwa laboratoire bwo kumenya HV burimo kwigunga virusi, PCR, ELISA ya serologiya, hamwe na immunoassays ya zahabu.

Culture Umuco wa virusi na PCR bitanga umwihariko ariko biratwara igihe, bisaba tekiniki, kandi bisaba ibikoresho bya laboratoire bigezweho, bikagabanya imikoreshereze yabyo.

Micro Micro-immunofluorescence (MIF) itanga ubunyangamugayo bwiza ariko bisaba microscope ya fluorescence hamwe nubusobanuro bwinzobere, bikabuza gushyira mubikorwa bisanzwe.

● ELISA na colloidal zahabu yerekana ko ikoreshwa cyane mubuvuzi bitewe n'ubworoherane, umuvuduko, ibyiyumvo byinshi kandi byihariye, hamwe no koroshya icyegeranyo (serumu / plasma).

Imikorere y'ibicuruzwa

Ibiranga HV-IgM / IgG (ELISA) Beier Bio

Type Ubwoko bw'icyitegererezo: Serumu, plasma

Icyitegererezo Cyicyitegererezo: Byombi IgM na IgG bavuga ko bakoresha iriba ryumwimerere hamwe na 1:11 (100µl sample diluent + 10µl sample), koroshya akazi no kugabanya imirimo yabakozi

Reagent Yiteguye-Gukoresha: Reagent zose ziriteguye usibye gukaraba (20 × yibanze). Ibara-kode kugirango imenyekane byoroshye

Procedure Uburyo bwo Kwiyongera: 30 min / 30 min / 15 min; mu buryo bwikora

Ection Kumenya Umuhengeri: 450 nm hamwe na 630 nm yerekanwe

Ips Ibipfundikizo bifunze: 96 cyangwa 48 amariba yameneka, buri kimwe gifite ibicuruzwa byacapwe kugirango bikurikiranwe kandi byoroshye

Beier Bio's HV-IgM / IgG (Zahabu ya Colloidal) Suzuma Ibiranga

Type Ubwoko bw'icyitegererezo: Serumu

Time Igihe cyo Kumenya: Ibisubizo mu minota 15; nta bikoresho by'inyongera bikenewe; nibyiza kwisuzumisha byihuse mubitaro, byihutirwa, kandi bitatanye abarwayi

● Inzira: Ongeraho 10µl icyitegererezo ku ikarita yikizamini neza ukoresheje igitonyanga; gusobanura ibisubizo muminota 15-20

Imikorere ya Clinical ya HV-IgM (ELISA), HV-IgG (ELISA), na HV-IgM / IgG (Zahabu ya Colloidal)