Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

Iki gikoresho kigaragaza anti-cyclic citrullinated peptide antibodies (CCP antibodies) mu byitegererezo bya serumu byabantu hashingiwe ku buryo butaziguye, hamwe na antigene ya peptide isukuye citrullinated peptide ikoreshwa nka antigen yo gutwikira.

Igikorwa cyo kwipimisha gitangirana no kongeramo serumu kumasoko ya reaction yabanje gushyirwaho hamwe na antigene zahanaguwe haruguru, zikurikirwa nigihe cyo gukora. Muri iyi incububasi, niba antibodiyite za CCP zihari murugero, bazamenya byumwihariko kandi bahuze na anticens ya cyclic citrullinated peptide yometse kuri microwells, bigakora antigen-antibody ihamye. Kugirango hamenyekane neza intambwe zikurikiraho, ibice bitavanze mumariba ya reaction bivanwa muburyo bwo gukaraba, bifasha gukuraho inzitizi ziterwa nibindi bintu biri muri serumu.

Ibikurikira, enzyme conjugate yongewe kumariba ya reaction. Nyuma yubushakashatsi bwa kabiri, iyi enzyme conjugate izahuza byumwihariko na antigen-antibody ihari, ikora urwego runini rwumubiri rurimo antigen, antibody, na enzyme conjugate. Iyo TMB substrate igisubizo cyinjijwe muri sisitemu, enzyme muri conjugate itera reaction yimiti hamwe na TMB substrate, bikavamo ihinduka ryibara rigaragara. Imbaraga zibi bibara zifitanye isano itaziguye nubunini bwa antibodiyite za CCP ziboneka muri serumu yumwimerere. Hanyuma, microplate umusomyi akoreshwa mugupima kwinjiza (Agaciro) kuvanga reaction. Iyo usesenguye agaciro kinjira, urwego rwa antibodiyite za CCP murugero rushobora kugenwa neza, rutanga urufatiro rwizewe rwo gupima no gusuzuma indwara.