Toxoplasma IgG ELISA Kit

Iki gikoresho cyerekana antibody ya Toxoplasma lgG (TOX-lgG) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antigen ya Toxoplasma.Nyuma yo kubanza kongeramo serumu cyangwa plasma kugirango bisuzumwe, bihuye na antibodi zihariye (TOX-lgG-Ab & lgM-Ab) ziboneka mubigereranyo byabarwayi zihuza na antigene mugice gikomeye, nibindi bice bidafunze bizakurwaho no gukaraba.Mu ntambwe ya kabiri, HRP (horseradish peroxidase) -guhuza anti-muntu lgG izitwara gusa hamwe na antibodi ya TOX lgG.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (TOX Ag) - (TOX-lgG) - (anti-muntu lgG-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango iteze imbere amabara, kandi HRP ihujwe nurwego rutera amabara abateza imbere reaction kubyara ibintu byubururu, ongeramo 50 µ I yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya TOX-lgG muri sample byagenwe numusomyi wa microplate.