Umuntu Cytomegalovirus IgM ELISA Kit

Iki gikoresho cyerekana antibody ya muntu Cytomegalovirus IgM (HCMV-IgM) muri serumu yumuntu cyangwa plasma ya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine ​​immunoglobuline M (anti-µ urunigi).Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodies za IgM murugero zirashobora gufatwa, nibindi bice bidafunze (harimo na antibodies zihariye za IgG) bizakurwaho no gukaraba.Intambwe ya kabiri, HRP (horseradish peroxidase)-antigene ya antijens izakora gusa na antibodiyite za HCMV IgM.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti- µ) - (HCMV-lgM) - (HCMV Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango amabara atere imbere, kandi HRP ihujwe nikigo itera abategura amabara kubyitwaramo. kubyara ibintu byubururu, ongeramo 50 µI yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya HCMV-IgM muri sample byagenwe numusomyi wa microplate.