Virusi ya Rubella IgM ELISA Kit

Iki gikoresho cyerekana virusi ya Rubella IgM antibody (RV-IgM) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine ​​ya immunoglobuline M (urunigi rwa anti-µ).Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodies za IgM murugero zirashobora gufatwa, nibindi bice bidafunze (harimo na antibodies zihariye za IgG) bizakurwaho no gukaraba.Mu ntambwe ya kabiri, HRP (horseradish peroxidase)-antigene ya antijene izakora gusa hamwe na antibodi ya RV IgM gusa.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti- µ) - (RV-lgM) - (RV Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango itere imbere amabara, kandi HRP ihujwe nurusobekerane itera abategura amabara kubyitwaramo. kubyara ibintu byubururu, ongeramo 50 µI yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya RV-IgM muri sample byagenwe numusomyi wa microplate.