Anti-Insuline (INS) Antibody ELISA Kit

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa muburyo bwa vitro bwo kumenya antibodiyide zirwanya insuline muri serumu yabantu.

Mubantu basanzwe, kuba antibodiyite za insuline mumaraso bituma bakunda kurwara Diyabete yo mu bwoko bwa 1 (T1DM). Antibodiyite zirwanya insuline zirashobora gukorwa kubera kwangirika kw ingirabuzimafatizo, bityo kubimenya bishobora kuba ikimenyetso cyimvune ya autoimmune β-selile. Nibimenyetso byambere byubudahangarwa bugaragara mubana bafite ibyago byinshi bya T1DM, kandi birashobora gukoreshwa mugutahura hakiri kare no gukumira T1DM, ndetse no gutanga inama zimwe na zimwe zo gusuzuma no gutangaza T1DM.

Kuba antibodiyite zihari mumaraso nimpamvu ikomeye yo kurwanya insuline. Abarwayi ba diyabete bahabwa imiti ya insuline barashobora kurwanya insuline bitewe no gukora antibodiyite za insuline, zirangwa no kongera urugero rwa insuline ariko kugenzura amaraso glucose bidashimishije. Muri iki gihe, antibodies za insuline zigomba gupimwa; ibisubizo byiza cyangwa titeri yiyongereye birashobora kuba ibimenyetso bifatika birwanya insuline. Byongeye kandi, uku gutahura bigira uruhare runini mugupima indwara ya Insuline Autoimmune (IAS).

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ihame

Iki gikoresho kigaragaza antibodiyide zirwanya insuline (IgG) mu ngero za serumu zabantu zishingiye ku buryo butaziguye, hamwe na insuline ya recombinant yanduye ya insuline ikoreshwa nka antigen yo gutwikira.

Igikorwa cyo kwipimisha gitangirana no kongeramo serumu icyitegererezo kumariba yabanje gutwikwa na antigen, hagakurikiraho incubation. Niba antibodiyite za insuline zihari murugero, zizahuza cyane na insuline yomuntu ya recombinant yomuntu mumariba, ikore antigen-antibody ihamye.

Nyuma yo gukaraba kugirango ukureho ibintu bidafunze kandi wirinde kwivanga, enzyme conjugate yongewe kumariba. Intambwe ya kabiri ya incubation yemerera izo enzyme guhuza cyane cyane na antigen-antibody ihari. Iyo TMB substrate igisubizo yatangijwe, reaction yibara ibaho munsi ya catalitiki yibikorwa bya enzyme murwego rugoye. Hanyuma, umusomyi wa microplate akoreshwa mugupima iyinjizwa (Agaciro), ituma hamenyekana ko hariho antibodiyide zirwanya insuline murugero.

Ibiranga ibicuruzwa

Ubukangurambaga bukabije, umwihariko no gutuza

Kugaragaza ibicuruzwa

Ihame	Enzyme ihuza immunosorbent assay
Andika	Ku buryo butaziguyeUburyo
Icyemezo	NMPA
Ingero	Serumu yumuntu / plasma
Ibisobanuro	48T /96T
Ubushyuhe bwo kubika	2-8℃
Ubuzima bwa Shelf	12amezi