Anti-Ovarian (AO) Antibody ELISA Kit

Ibisobanuro bigufi:

Intanga ngore irimo amagi, zona pellucida, selile granulosa, nibindi, mubyiciro bitandukanye byiterambere. Buri kintu cyose gishobora gutera antibodiyite zirwanya ovarian (AoAb) kubera imvugo idasanzwe ya antigen. Ovarian antigen isuka iterwa no gukomeretsa kw'intanga, kwandura, cyangwa gutwika bishobora gutera AoAb mubantu bafite ubudahangarwa bw'umubiri. AoAb yangiza intanga ngore kandi ikangiza imikorere ya nyababyeyi na nyababyeyi, itera ubugumba no gukuramo inda.

AoAb yabonetse bwa mbere mu barwayi bafite intanga ngore (POF) na amenorrhea kare, ifitanye isano na autoimmune reaction. AoAb ubanza kugabanya uburumbuke kandi amaherezo biganisha ku kunanirwa kw'intanga. Abarwayi batabyara bafite AoAb nziza ariko nta POF ishobora guhura nibibazo byinshi bya POF, bisaba isuzuma ryintanga ngore.

AoAb positivite ni nyinshi mubarwayi batabyara kandi bakuramo inda, byerekana isano ya hafi. Ubushakashatsi bwerekana AoAb itera ubugumba kuruta gukuramo inda. Ubushakashatsi buherutse gukorwa bwerekana AoAb mu barwayi benshi ba PCOS, byerekana ko indwara ya ovarian yatewe na immunite na cytokine idasanzwe bishobora gutera PCOS n'uburumbuke, bikeneye ubushakashatsi bwimbitse.

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Ibicuruzwa birambuye

Ibicuruzwa

Ihame

Iki gikoresho kigaragaza antibodiyite zirwanya ovarian (IgG) mu ngero za serumu zabantu zishingiye ku buryo butaziguye, hamwe na antigene ya ovarian isukuye ikoreshwa mbere yo gutwikira microwells.

Igikorwa cyo kwipimisha gitangirana no kongeramo urugero rwa serumu kumariba ya antigen-precoated reaction ya incubation. Niba antibodiyite zirwanya intanga ngore ziboneka murugero, zizahuza cyane na antigens ya ovarian membrane yabanje gutwikirwa muri microwells, bigakora antigen-antibody ihamye. Ibice bidafunze noneho bivanwaho kugirango hamenyekane neza.

Ibikurikira, horseradish peroxidase (HRP) -yanditseho imbeba irwanya abantu IgG antibodiyite zongerewe kumariba. Nyuma yubushakashatsi bwa kabiri, antibodiyite zanditseho enzyme zihuza cyane na antibodiyide zirwanya ovarian munganda zisanzwe za antigen-antibody, zikora "antigen-antibody-enzyme label" yuzuye.

Hanyuma, TMB substrate igisubizo cyongeyeho. HRP muri complexe itera imiti yimiti hamwe na TMB, itanga ibara rigaragara. Kwinjiza (Agaciro) k'igisubizo cya reaction bipimwa hifashishijwe umusomyi wa microplate, kandi kuba antibodiyite zirwanya ovarian zihari cyangwa zidahari ziteganijwe hashingiwe ku gisubizo cyo gukuramo.

Ibiranga ibicuruzwa

Ubukangurambaga bukabije, umwihariko no gutuza

Kugaragaza ibicuruzwa

Ihame	Enzyme ihuza immunosorbent assay
Andika	Ku buryo butaziguyeUburyo
Icyemezo	NMPA
Ingero	Serumu yumuntu / plasma
Ibisobanuro	48T /96T
Ubushyuhe bwo kubika	2-8℃
Ubuzima bwa Shelf	12amezi