Enterovirus 71 (EV71) IgG ELISA Kit
Ihame
Iki gikoresho cyerekana Enterovirus 71 IgG antibody (EV71-IgG) muri serumu yumuntu cyangwa plasma yintangarugero, imirongo ya microwell ya polystirene yabanje gushyirwaho antigen ya Anterovirus 71.Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodi zihariye (EV71-IgG-Ab & zimwe IgM-Ab) ziboneka mubigereranyo byabarwayi zihuza na antigene mugice gikomeye, nibindi bice bidafunze bizakurwaho no gukaraba.Intambwe ya kabiri, HRP (horseradish peroxidase) -guhuza anti-muntu IgG izitwara gusa na antibodiyite za EV71 IgG.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (EV71 Ag) - (EV71-IgG) - (anti-muntu IgG-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongeweho kugirango iterambere ryamabara, kandi HRP ihujwe nurwego rutera amabara abateza imbere reaction kubyara ibintu byubururu, ongeramo 50μL yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya EV71-IgG muri sample byagenwe numusomyi wa microplate.
Ibiranga ibicuruzwa
Ubukangurambaga bukabije, umwihariko no gutuza
Kugaragaza ibicuruzwa
Ihame | Enzyme ihuza immunosorbent assay |
Andika | Uburyo butaziguye |
Icyemezo | CE |
Ingero | Serumu yumuntu / plasma |
Ibisobanuro | 48T / 96T |
Ubushyuhe bwo kubika | 2-8 ℃ |
Ubuzima bwa Shelf | Amezi 12 |
Gutegeka Amakuru
Izina RY'IGICURUZWA | Gupakira | Ingero |
Enterovirus 71 (EV71) IgG ELISA Kit | 48T / 96T | Serumu yumuntu / plasma |