Enterovirus 71 (EV71) IgM ELISA Kit

Ibisobanuro bigufi:

Enterovirus 71 IgM (EV71-IgM) ELISA kit ni enzyme ifitanye isano na immunosorbent igamije kumenya neza antibodi zo mu rwego rwa IgM kuri Enterovirus 71 muri serumu cyangwa plasma.Igenewe gukoreshwa muri laboratoire y’amavuriro mu gusuzuma no gucunga abarwayi bajyanye no kwandura Enterovirus 71.

Umuntu enterovirus 71 (EV71), umunyamuryango mushya wa Enteroviridae, ni impamvu itera indwara y'intoki, ibirenge ndetse no mu kanwa (HFMD) kandi rimwe na rimwe ikaba ifitanye isano n'indwara zikomeye zo mu mitsi yo hagati.Indwara zikunze kwiyongera muri Kamena na Nyakanga.Kwandura na EV71 birashobora kuba bidafite ibimenyetso cyangwa bishobora gutera impiswi no kurwara.Abana bari munsi yimyaka itanu bafite ibyago byinshi byo kwandura indwara zikomeye kurusha abantu bakuru ndetse nabana bakuru.

EV71 yanduza umuntu ku muntu binyuze mu buryo butaziguye kandi binyuze mu kwanduza amaboko cyangwa ibintu byanduye umwanda.Gusohora kw'izuru n'umuhogo, amacandwe cyangwa amazi ava mu bisebe nabyo bishobora gukwirakwiza virusi.

EV71 irashobora kwigunga no kumenyekana mu muhogo no ku ntebe ukoresheje ibizamini bya PCR.Virusi RNA yagaragaye mumazi yuruhu rwamaraso, mumaraso ninkari.Kwisuzumisha mbere kandi byoroshye birashobora kugerwaho ukoresheje EV71 yihariye ya serologiya, harimo ibizamini byihariye bya antibody ya IgM.

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Ibicuruzwa birambuye

Ibicuruzwa

Ihame

Iki gikoresho cyerekana Enterovirus 71 IgM antibody (EV71-IgM) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine za immunoglobuline M (anti-μ chain) .Nyuma yo kubanza kongeramo serumu cyangwa plasma kugirango bibe. hasuzumwe, antibodies za IgM murugero zishobora gufatwa, nibindi bice bidafitanye isano (harimo na antibodi yihariye ya IgG) bizakurwaho no gukaraba.Intambwe ya kabiri, HRP (horseradish peroxidase)-antigene ya antijene izakora gusa na antibodiyite za EV71 IgM.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango iteze imbere amabara, kandi HRP ihujwe nikigo itera abaterankunga amabara. kubyara ibintu byubururu, ongeramo 50μL yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya EV71-IgM muri sample byagenwe numusomyi wa microplate.

Ibiranga ibicuruzwa

Ubukangurambaga bukabije, umwihariko no gutuza

Kugaragaza ibicuruzwa

Ihame	Enzyme ihuza immunosorbent assay
Andika	Uburyo bwo gufata
Icyemezo	CE
Ingero	Serumu yumuntu / plasma
Ibisobanuro	48T / 96T
Ubushyuhe bwo kubika	2-8 ℃
Ubuzima bwa Shelf	Amezi 12