Virusi ya Hepatitis D IgM ELISA Kit

Iki gikoresho kigaragaza virusi ya hepatite D IgM antibody (HDV-IgM) muri serumu yumuntu cyangwa plasma yintangarugero, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine ​​za immunoglobuline M (anti-μ chain).Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodies za IgM murugero zirashobora gufatwa, nibindi bice bidafunze (harimo na antibodies zihariye za IgG) bizakurwaho no gukaraba.Mu ntambwe ya kabiri, HRP (horseradish peroxidase)-antigene ya antijene izakora gusa na antibodiyite ya HDV IgM.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango iteze imbere amabara, kandi HRP ihujwe nurwego rutera amabara abitegura gukora. kubyara ibintu byubururu, ongeramo 50μl yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya HDV-IgM muri sample byagenwe numusomyi wa microplate.