Virusi ya Hepatitis E IgM ELISA Kit
Ihame
Iki gikoresho cyerekana virusi ya hepatite E IgM antibody (HEV-IgM) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine za immunoglobuline M (urunigi rwa anti-μ).Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodies za IgM murugero zirashobora gufatwa, nibindi bice bidafunze (harimo na antibodies zihariye za IgG) bizakurwaho no gukaraba.Intambwe ya kabiri, HRP (horseradish peroxidase)-antigens ya antigene izakora gusa na antibodiyite ya HEV IgM.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango amabara atere imbere, kandi HRP ihujwe nurwego rutera amabara abiteza imbere. kubyara ibintu byubururu, ongeramo 50μl yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya HEV-IgM muri sample byagenwe numusomyi wa microplate.
Ibiranga ibicuruzwa
Ubukangurambaga bukabije, umwihariko no gutuza
Kugaragaza ibicuruzwa
Ihame | Enzyme ihuza immunosorbent assay |
Andika | Uburyo bwo gufata |
Icyemezo | CE |
Ingero | Serumu yumuntu / plasma |
Ibisobanuro | 48T / 96T |
Ubushyuhe bwo kubika | 2-8 ℃ |
Ubuzima bwa Shelf | Amezi 12 |
Gutegeka Amakuru
Izina RY'IGICURUZWA | Gupakira | Ingero |
Virusi ya Hepatitis E IgM ELISA Kit | 48T / 96T | Serumu yumuntu / plasma |