Kurwanya SARS-CoV-2 Kutabogama Ikizamini cya Antibody (ELISA)

Anti-SARS-CoV-2 Kutabogama Ibizamini bya Antibody (ELISA) Ishusho Yerekanwe

Ibisobanuro bigufi:

Iki gicuruzwa gikoresha enzyme ihujwe na immunosorbent kugirango isuzume neza antibodi zose ziri muri serumu na plasma zishobora guhungabanya imikoranire ya RBD-ACE2, kandi ubu buryo ntabwo bugarukira gusa kubwoko bwa genera hamwe na antibody.Kutabogama antibodiyite birashobora guhagarika virusi ya spike glycoprotein reseptor ihuza akarere (RBD) kudakorana na reseptor angiotensin-ihindura enzyme-2 (ACE2).

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ihame

Igikoresho gikoresha ihame ryamarushanwa kugirango hamenyekane antibodiyite zidafite aho zibogamiye kuri SARS-CoV-2 muri serumu cyangwa plasma.Ubwa mbere, icyitegererezo kigomba gupimwa, kugenzura neza, no kugenzura nabi bivanze na HRP-RBD kugirango antibody itabogamye ihuza HRP-RBD, hanyuma imvange ikongerwaho ku isahani yo gufata mbere yashizwemo proteine ya HACE2.HRP-RBD itagomba guhagarika antibody kimwe na HRP-RBD ihujwe na antibody idafite aho ibogamiye izafatirwa ku isahani isize, mu gihe ikigo cya HRP-RBD kigomba kubuza antibody gukuraho igihe cyo gukaraba.TMB igisubizo noneho cyongeweho kugirango gitezimbere ibara.Hanyuma, guhagarika igisubizo byongeweho kandi reaction yararangiye.Kubaho cyangwa kutabaho kwa Anti-SARS-CoV-2 itabuza antibody mu cyitegererezo byaciwe no kwinjiza (A-agaciro) kubushakashatsi bwa microplate.

Ibiranga ibicuruzwa

Ubukangurambaga bukabije, umwihariko no gutuza

Kugaragaza ibicuruzwa

Ihame	Enzyme ihuza immunosorbent assay
Andika	Uburyo bwo guhatana
Icyemezo	CE
Ingero	Serumu yumuntu / plasma
Ibisobanuro	96T
Ubushyuhe bwo kubika	2-8 ℃
Ubuzima bwa Shelf	Amezi 12