Mycoplasma Umusonga IgM ELISA Kit

Iki gikoresho kigaragaza Mycoplasma Pneumoniae IgM (MP-IgM) muri serumu yumuntu cyangwa plasma yintangarugero, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodi zerekeza kuri proteine ​​za immunoglobuline M (anti-μ chain) .Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa. , antibodies za IgM murugero zirashobora gufatwa, nibindi bice bidafunze (harimo na antibodi yihariye ya IgG) bizakurwaho no gukaraba.Mu ntambwe ya kabiri, HRP (horseradish peroxidase) -imisigiti ya antijene izakora gusa na antibodiyite MP IgM.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango iteze imbere amabara, kandi HRP ihujwe nurwego rutera amabara abiteza imbere. kubyara ibintu byubururu, ongeramo 50μl yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya MP-IgM muri sample byagenwe numusomyi wa microplate.