Indwara y'iseru (MV) IgG ELISA Kit

Iki gikoresho kigaragaza virusi ya Measles IgG antibody (MV-IgG) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antigen virusi ya Measles.Nyuma yo kubanza kongeramo serumu cyangwa plasma bigomba gusuzumwa, antibodi zihariye (MV-IgG-Ab & IgM-Ab) ziboneka mubigereranyo byabarwayi zihuza na antigene mugice gikomeye, nibindi bice bidafunze bizakurwaho no gukaraba.Intambwe ya kabiri, HRP (horseradish peroxidase) -guhuza anti-muntu IgG izitwara gusa na antibodi za MV IgG gusa.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (MV Ag) - (MV-IgG) - (anti-muntu IgG-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango amabara atere imbere, kandi HRP ihujwe nikigo itera abateza imbere amabara. kubyara ibintu byubururu, ongeramo 50μl yo guhagarika igisubizo, hanyuma uhinduke umuhondo. Kubaho kwinjiza antibody ya MV-IgG muri sample byagenwe numusomyi wa microplate.