SARS-COV-2 Igiteranyo Cyuzuye Ab Ikizamini (Elisa)

SARS-CoV-2 Igipimo Cyuzuye Ab Ikizamini (ELISA) gishingiye ku isuzuma rya immunoenzymatic kugirango hamenyekane antibodiyite za SARS-CoV-2 muri serumu yumuntu, plasma (EDTA, Heparin cyangwa sodium citrate).Amasahani ya micro nk'icyiciro gikomeye yashizwemo na recombinant SARS-CoV-2 recombinant Receptor Binding Domain protein.Muntambwe yambere ya incubation ihuye na antibodies zihariye (SARS-CoV-2-IgG-Ab & bamwe IgM-Ab) igaragara mubigereranyo byabarwayi ihuza na antigene mugice gikomeye.Kurangiza inkubasi ibice bidahujwe byogejwe.Ku ntambwe ya kabiri ya incubation SARS-CoV-2 recombinant Receptor Binding Domain protein conjugate (SARS-CoV-2 recombinant Receptor Binding Domain protein peroxidase conjugate) hiyongereyeho cyane cyane antibodiyite za SARS-CoV-2 (harimo IgG na IgM) bivamo ishingwa rya immunocomplexes isanzwe.Nyuma yo gukaraba kabiri kugirango ukureho conjugate irenze, TMB / Substrate yongeyeho (intambwe3).Ibara ry'ubururu rikura rihinduka umuhondo nyuma yo guhagarika reaction hamwe no guhagarika igisubizo.Kwinjira kwa kalibuteri hamwe nicyitegererezo bigenwa no gukoresha micro plaque ya ELISA.Ibisubizo kuburugero rwabarwayi biboneka ugereranije nagaciro kagabanijwe.