Ikizamini cyo Gusuzuma TB-IGRA

TB-IGRA Ikizamini cyo Gusuzuma Ikiranga Ishusho

Ibisobanuro bigufi:

Ikizamini cyo gusuzuma indwara ya TB-IGRA, nanone cyitwa Interferon Gamma Release Assay, ni ELISA yo kumenya umubare wa Interferon Gamma (IFN-γ) isubiza muri vitro itera imbaraga za Mycobacterium Tuberculose antigens mu maraso y'abantu.Igituntu-IGRA gipima ubudahangarwa bw'umuntu kuri Mycobacterium Igituntu.Ikizamini kigenewe gukoreshwa nk'imfashanyo mu gusuzuma indwara yanduye igituntu harimo n'indwara y'igituntu yihishe ndetse n'indwara y'igituntu.

Ubudahangarwa bw'umubiri buterwa na Mycobacterium igituntu ni igisubizo cya selile.Nyuma yo kwandura igituntu cya Mycobacterium, umubiri utanga selile yihariye yibuka T izenguruka mumaraso ya peripheri.Ibipimo bya IFN-γ kurwanya Mycobacterium igituntu byagaragaye ko ari tekinike nziza yo kumenya indwara yanduye igituntu (haba mu bwihisho kandi ikora), yitwa IFN-γ mu gusohora vitro (IGRA).Itandukaniro rikomeye riterwa nigeragezwa ryigituntu (TST) nuko IGRA ihitamo antigene yihariye igaragara gusa muri Mycobacterium igituntu ariko ikaba idahari muri BCG na mycobacteria itari igituntu.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ihame

Igikoresho gikoresha interferon-γ kurekura indwara ya Mycobacterium igituntu (TB-IGRA) kugirango ipime ubukana bwimikorere yumubiri wa selile yunganirwa na Mycobacterium tuberculose antigen yihariye.
Enzyme ihuza immunosorbent assay hamwe na antibody ya sandwich ya kabiri.
• Microplates zabanje gushyirwaho antibodiyite zirwanya IFN-γ.
• Ingero zigomba gupimwa zongewe mumariba ya antibody yometse kuri microplate, hanyuma peroxidase ya horseradish (HRP) -conjugated anti IFN-γ antibodies zongerwa mumariba yabigenewe.
• IFN-γ, niba ihari, izakora sandwich igizwe na antibodiyite za IFN-γ na HRP-conjugated anti IFN-γ antibodies.
• Ibara rizatezwa imbere nyuma yo kongeramo ibisubizo, kandi bizahinduka nyuma yo kongera ibisubizo bihagarara.Kwinjira (OD) bipimwa numusomyi wa ELISA.
• IFN-γ kwibanda muri sample bifitanye isano na OD yagenwe.

Ibiranga ibicuruzwa

ELISA isuzumisha neza kwandura igituntu cyihishe kandi gikora

Nta kwivanga mu rukingo rwa BCG

Kugaragaza ibicuruzwa

Ihame	Enzyme ihuza immunosorbent assay
Andika	Uburyo bwa Sandwich
Icyemezo	CE ， NMPA
Ingero	Amaraso yose
Ibisobanuro	48T (menya ingero 11);96T (menya ingero 27)
Ubushyuhe bwo kubika	2-8 ℃
Ubuzima bwa Shelf	Amezi 12