Herpes Simplex II IgM ELISA Kit

Iki gikoresho kigaragaza virusi ya Herpes Simplex II IgM antibody (HSV2-IgM) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine ​​ya immunoglobuline M (anti-µ urunigi).Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodies za IgM murugero zirashobora gufatwa, nibindi bice bidafunze (harimo na antibodies zihariye za IgG) bizakurwaho no gukaraba.Mu ntambwe ya kabiri, HRP (horseradish peroxidase) -imisigiti ya antijene izakora gusa na antibodiyite ya HSV2 IgM.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti- µ) - (HSV2-lgM) - (HSV2 Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongewemo kugirango iterambere ryamabara, kandi HRP ihujwe nurwego rutera amabara abitegura gukora. kubyara ibintu byubururu, ongeramo 50 µ I yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya HSV2-IgM muri sample byagenwe numusomyi wa microplate.