Herpes Simplex virusi I IgM ELISA Kit

Iki gikoresho cyerekana Herpes Simplex Virus I lgM antibody (HSV1-lgM) muri serumu yumuntu cyangwa icyitegererezo cya plasma, imirongo ya microwell ya polystirene yabanje gushyirwaho antibodiyide zerekeza kuri proteine ​​za immunoglobuline M (anti-µ urunigi).Nyuma yo kubanza kongeramo serumu cyangwa plasma zigomba gusuzumwa, antibodies za lgM murugero zirashobora gufatwa, nibindi bice bidafitanye isano (harimo na antibodies zihariye za lgG) bizakurwaho no gukaraba.Mu ntambwe ya kabiri, HRP (horseradish peroxidase)-antigene ya antijene izakora gusa na antibodies za HSV1 lgM gusa.Nyuma yo gukaraba kugirango ukureho HRP-conjugate idafunze, ibisubizo bya chromogene byongewe mumariba.Imbere ya (anti- µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, nyuma yo koza isahani, insimburangingo ya TMB yongeweho kugirango iterambere ryamabara, kandi HRP ihujwe nikigo itera abateza imbere amabara kubyitwaramo. kubyara ibintu byubururu, ongeramo 50 µ I yo guhagarika igisubizo, hanyuma uhindure umuhondo.Kubaho kwa antibody ya HSV1-lgM muri sample byagenwe numusomyi wa microplate.